Title

Glycan based detection and drug susceptibility of influenza virus

Authors

Hieu Dinh, Georgia State UniversityFollow
Xiaohu Zhang, Georgia State UniversityFollow
Joyce Sweeney, Georgia State UniversityFollow
Yang Yang, Georgia State UniversityFollow
Yun He, Georgia State UniversityFollow
Abasaheb Dhawane, Georgia State UniversityFollow
Suri S. Iyer, Georgia State UniversityFollow

Document Type

Article

Publication Date

2014

Abstract

ABSTRACT: We have developed a panel of synthetic glycans as receptor mimics for the specific capture of influenza viruses. The glycans were printed onto commercial glass slides using a free amine at the end of a spacer to generate a small focused microarray. The microarray was evaluated for its ability to capture three different strains of influenza A virus, two H1N1, A/Brisbane/59/2007 and A/Solomon Islands/3/2006 and one H3N2, A/Aichi/2/1968. We observed an excellent detection ability with some compounds exhibiting clinically relevant (101 plaque forming units) limit of detection. We also tested the drug susceptibility of current antivirals, Zanamivir and Ostelamivir using this microarray and could determine antiviral resistance for these strains.

Comments

Originally published in:

Anal Chem, 86 (16), 8238-44. DOI: 10.1021/ac501624v

This is an open access article published under an ACS AuthorChoice License, which permits copying and redistribution of the article or any adaptations for non-commercial purposes.

Recommended Citation

Dinh, Hieu; Zhang, Xiaohu; Sweeney, Joyce; Yang, Yang; He, Yun; Dhawane, Abasaheb; and Iyer, Suri S., "Glycan based detection and drug susceptibility of influenza virus" (2014). Chemistry Faculty Publications. 9.
https://scholarworks.gsu.edu/chemistry_facpub/9