Date of Award
4-19-2010
Degree Type
Thesis
Degree Name
Master of Science (MS)
Department
Chemistry
First Advisor
Dr. Jenny J. Yang - Committee Chair
Second Advisor
Dr. Aimin Liu - Committee Member
Third Advisor
Dr. Zhi-Ren Liu - Committee Member
Abstract
More than one-third of all Magnetic Resonance Imaging (MRI) scans employ image-enhancing contrast agents to increase the differential signal intensity between diseased and normal tissue. Because current clinical contrast agents exhibit low relaxivity (mM-1 s-1), low dose efficiency, and rapid secretion, we have designed a group of protein-based MRI contrast agents with multiple gadolinium binding sites. In this study, the developed purification method for Class ProCA-3 agents allows for a quick and cost-effective way to abstract up to 109 mg of pure, soluble protein from a 1L E. Coli cell pellet devoid of DNA or RNA “contamination” for extensive animal studies. Circular dichroism far-UV spectra ensure the metal stability of the agents, revealing maintenance of their native α-helical structure in the presence and absence of metal ions. Furthermore, substantial evidence supports the high dose efficiency of these agents, exhibiting up to five folds higher relaxivity than their analogous commercial competitors.
DOI
https://doi.org/10.57709/1350346
Recommended Citation
Hubbard, Kendra Lynette, "Purification and Structural Characterization of a Novel Class of Protein- Based Magnetic Resonance Imaging Contrast Agents." Thesis, Georgia State University, 2010.
doi: https://doi.org/10.57709/1350346