Date of Award
12-17-2014
Degree Type
Thesis
Degree Name
Master of Science (MS)
Department
Chemistry
First Advisor
Zhen Huang
Second Advisor
Stuart A. Allison
Third Advisor
Kathryn Grant
Abstract
Nucleic acids are important bio-macromolecules in living systems. They are involved in important functions like gene expression and regulation. Nucleoside triphosphates serve as precursors for biochemical synthesis of modified nucleic acids and nucleotide coenzymes. The modification of nucleic acids, particularly at nucleobases, can expand the function and chemical properties of nucleic acid. Herein, we report the chemical synthesis of a novel thiol-modified nucleoside S-(3-(acetylthio)propyl)-5-(mercaptomethyl)-uridine and the corresponding nucleotide via a “new synthetic methodology” developed in our laboratory. The synthesized triphosphate was used for RNA transcription. The activity and nuclease resistance of the transcribed RNA is studied. The results showed that the properties of the nucleotide with thiol functionality are as good as the native. The modified RNA can be used for RNA/protein complex structure studies and gold nanoparticles stabilizer. They can also serve as a probe in DNA/RNA microchip surface functionalization for detection of various diseases and pathogens.
DOI
https://doi.org/10.57709/6428085
Recommended Citation
Esmaeili, Razieh, "Synthesis and Biochemical Studies of a Novel Thiol Modified Nucleotide." Thesis, Georgia State University, 2014.
doi: https://doi.org/10.57709/6428085