Date of Award

5-10-2017

Degree Type

Dissertation

Degree Name

Doctor of Philosophy (PhD)

Department

Mathematics and Statistics

First Advisor

Vladimir E. Bondarenko

Second Advisor

Igor Belykh

Third Advisor

Yaroslav Molkov

Fourth Advisor

Alexandra Smirnova

Abstract

The β1-adrenergic signaling system is one of the most important systems regulating heart function. Activation of this system leads to an increased heart rate, which can be beneficial during exercise, but can lead to cardiac hypertrophy and heart failure with continuous over-stimulation. In this dissertation, we have developed two comprehensive mathematical models of mouse ventricular myocyte contraction. The first model is based on a previously published mathematical model of action potential and Ca2+ handling mechanism of the mouse cardiac cell that are not modulated by the β1-adrenergic signaling system. The model was verified with experimental data on mouse myocyte contraction at room temperature. In the model, we implement simplified sarcomere length variability and indirect modulation of the tropomyosin transition rates by Ca2+ and troponin. The resulting model describes well steady-state force-calcium relationships, dependence of contraction force on sarcomere length, time course of contraction force and myocyte shortening, frequency dependence of contraction force and cellular contraction, and experimentally measured derivatives of myocyte length variation. We emphasize the importance of including variable sarcomere length in the model for ventricular myocyte contraction and investigate the differences in contraction force and cell shortening for epicardial and endocardial ventricular myocytes. The second model of the mouse ventricular myocyte contraction includes a more advanced description of the forces involved in myocyte contraction (active, passive, viscous, and flexible forces) and the β1-adrenergic signaling system. The model was verified by the simulation of major experimental protocols on measurements of steady-state force-calcium relationships, crossbridge release rate (krel) and force development rate (kdf), force-velocity relationship, and force redevelopment rate (ktr). It also reproduces quite well frequency and isoproterenol dependencies for [Ca2+]i transients, total contraction force, and sarcomere shortening. The resulting mathematical model reveals the mechanisms of increased contraction force and myocyte shortening upon stimulation of β1-adrenergic receptors. The developed mathematical models can be used further for simulations of contraction of ventricular myocytes from genetically modified mice and myocytes from mice which have developed chronic cardiac diseases.

Available for download on Wednesday, April 18, 2018

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