Date of Award
Master of Science (MS)
Zika virus (ZIKV) was declared as “Public Health Emergency of International Concern” in 2016. Differentiating ZIKV recent infections (< 12 weeks post symptom onset) from past infections (> 12 weeks post symptom onset) is a significant challenge of serological test, which is widely used to detect ZIKV infections. We developed ZIKV NS1 IgM and IgG capture ELISAs and applied four indicators: IgM values, capture IgM/IgG ratios, IgG avidity, combined IgG avidity and capture IgM/IgG ratios, to differentiate ZIKV recent and past infection for a cohort of serum collected in ZIKV epidemic regions of Bogota, Colombia. The sensitivity for differentiating ZIKV recent and past infections using each of the four indicators is: 54.78%, 54.88%, 40.68%, and 31.25%, respectively. The specificity for differentiating ZIKV recent and past infection using each of the four indicators is: 62.50%, 84.21%, 58.33%, and 47.37%, respectively.
Zhu, Jinjin, "Development of IgM and IgG capture ELISAs for differentiating ZIKV recent and past infection." Thesis, Georgia State University, 2021.
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