Date of Award

8-10-2021

Degree Type

Thesis

Degree Name

Master of Science (MS)

Department

Biology

First Advisor

Julia Hilliard

Second Advisor

David Katz

Third Advisor

Richard Dix

Abstract

Zika virus (ZIKV) was declared as “Public Health Emergency of International Concern” in 2016. Differentiating ZIKV recent infections (< 12 weeks post symptom onset) from past infections (> 12 weeks post symptom onset) is a significant challenge of serological test, which is widely used to detect ZIKV infections. We developed ZIKV NS1 IgM and IgG capture ELISAs and applied four indicators: IgM values, capture IgM/IgG ratios, IgG avidity, combined IgG avidity and capture IgM/IgG ratios, to differentiate ZIKV recent and past infection for a cohort of serum collected in ZIKV epidemic regions of Bogota, Colombia. The sensitivity for differentiating ZIKV recent and past infections using each of the four indicators is: 54.78%, 54.88%, 40.68%, and 31.25%, respectively. The specificity for differentiating ZIKV recent and past infection using each of the four indicators is: 62.50%, 84.21%, 58.33%, and 47.37%, respectively.

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