Unprecedented DNA Photocleavage Mediated by Quinolinium Pentamethine Carbocyanine Dyes in the NIR Wavelength Range
Date of Award
Master of Science (MS)
Kathryn B. Grant
Dabney White Dixon
Here we report the DNA photocleavage abilities of near-infrared (NIR) quinolinium pentamethine carbocyanine dyes substituted with either bromine, chlorine, or hydrogen at the meso-carbon position. We are interested in developing these cyanine dyes as treatment agents in photodynamic cancer therapy because they can be activated to selectively kill diseased cells with NIR light that deeply penetrates biological tissues. UV-visible, CD, and fluorescence spectra show that the brominated quinolinium cyanine dyes 4 and KI2 are stable in aqueous buffer and bind to DNA in a non-intercalative manner both externally and in the DNA minor groove. Reactions run in the presence and absence of fluorescent probes and chemical additives reveal that irradiation of dye 4 and KI2 with 707–759-nm and 830-905 nm NIR light respectively generates Type I hydroxyl radicals that form direct strand breaks in plasmid DNA in high yield.
Ahoulou, Effibe O., "Unprecedented DNA Photocleavage Mediated by Quinolinium Pentamethine Carbocyanine Dyes in the NIR Wavelength Range." Thesis, Georgia State University, 2021.
File Upload Confirmation
Available for download on Sunday, July 23, 2023