Date of Award
Summer 8-7-2012
Degree Type
Thesis
Degree Name
Master of Science (MS)
Department
Chemistry
First Advisor
Dr. Aimin Liu
Second Advisor
Dr. David Wilson
Third Advisor
Dr. Donald Hamelberg
Abstract
The transcription factor NF-κB interacts with many other co-regulator proteins that modulate its binding and transcriptional activity. One of these co-regulators, Pirin, is an iron-dependent metalloprotein that has been shown to enhance the DNA binding of NF-κB homodimers. Here, we characterize the interactions between Pirin and its known NF-κB binding partners and examined the role of Bcl-3, a protein that is required for Pirin’s interaction with p50. In addition, we use site-directed mutagenesis to alter conserved residues within Pirin’s metal binding environment and observed how it affected the DNA binding and conformation of the Pirin-NF-κB complex. These studies show that, while a similar enhancing effect on DNA binding is observed, the interactions of Pirin with different NF-κB members are distinct from each other and could possibly have different physiological purposes.
DOI
https://doi.org/10.57709/3099527
Recommended Citation
Rehmani, Imran J., "Studying the DNA Binding and Conformation of Metal-Binding Site Mutations in Pirin." Thesis, Georgia State University, 2012.
doi: https://doi.org/10.57709/3099527