This study presents the translation, cultural adaptation, and psychometric validation of the Bangla Grit-O scale for use among university students in Bangladesh and examines its measurement invariance across genders. The Grit-O scale, a widely used instrument for measuring grit, is a 12-item U.S.-originated measure with two constructs – Consistency of Interests (CI) and Perseverance of Effort (PE). Grit, defined as passion and perseverance toward long-term goals, predicts academic success. As a non-cognitive personality trait, grit may function differently in individualist versus collectivist cultures and vary across genders. Previous cross-cultural studies reported inconsistent factor structures, with limited examination of gender-based invariance. Bangladesh is a collectivist country, and the Grit-O scale has never been translated into its native language, Bangla. These gaps offer contexts to establish the Bangla Grit-O scale as a culturally and psychometrically sound measure. The Bangla Grit-O scale was transadapted following forward-backward translation processes. Using this transadapted Bangla Grit-O scale, data were collected from 389 Bangladeshi university students. Confirmatory factor analysis did not support the original 12-item two-factor model. Based on factor loadings and model diagnostics, four items were removed and four correlated residuals were specified. The resulting 8-item two-factor model, comprising four CI and four PE items, demonstrated strong model fit. Measurement invariance testing using multigroup confirmatory factor analysis supported full configural, metric, and scalar invariances across gender groups. By providing robust psychometric evidence for the Bangla Grit-O scale, this study positions it as a rigorous measurement instrument for future grit-relevant research in Bangladesh and cross-cultural comparisons.