Murine cytomegalovirus (MCMV) infection has been associated with activation of multiple programmed cell death pathways in experimental models of cytomegalovirus disease, but its relationship to parthanatos-associated signaling in microglial cells remains unclear. This study investigated whether MCMV infection alters expression of poly(ADP-ribose) polymerase-1 (PARP-1) and poly(ADP-ribose) (PAR) in BV-2 murine microglial cells. PARP-1 mRNA expression was measured by RT-qPCR at 1, 2, and 3 days post-infection, and PARP-1 and PAR proteins were examined by Western blot under multiple infection conditions. PARP-1 mRNA remained near baseline, and PARP-1 protein was detected in both mock-infected and infected cells without clear infection-associated upregulation. PAR accumulation was observed only at 3 days post-infection under the low-dose condition. These findings suggest that MCMV does not strongly induce parthanatos-associated signaling in BV-2 cells under the conditions tested, although limited late PAR-associated changes were detected.