Escapin is an L-amino acid oxidase that produces antimicrobial metabolites collectively called “Escapin Intermediate Products” (EIP-K). EIP-K and H₂O₂ together were previously shown to be bactericidal towards diverse planktonic bacteria. The present work investigates the ability of EIP-K and H₂O₂ to antagonize bacterial biofilms, using Pseudomonas aeruginosa as a model. The project had three aims: 1) determine the most effective concentrations of EIP-K and H₂O₂ necessary to break down existing P. aeruginosa biofilms, using a crystal violet assay; 2) examine the ability of EIP-K + H₂O₂ to inhibit biofilm formation, using triphenyl tetrazolium chloride dye; and 3) determine the effect of EIP-K + H₂O₂ on the viability, biomass and structure of biofilms cultivated in flow cells using confocal laser scanning microscopy (CLSM). Results showed that EIP-K + H₂O₂ significantly reduced biofilm biomass relative to controls and that the compounds are effective at nanomolar concentrations.