The aim of this study was to investigate the potentiation effect of a conditioning activity (CA) on a high velocity movement (HVM) that occurs in a different biomechanical plane. Previous studies have shown an increase in HVM performance following a CA that is biomechanically similar, but it is unknown whether this phenomenon will occur when the two movements (CA and HVM) are biomechanically dissimilar, i.e. occurs in a different movement plane. This study employed a within-subject, repeated measures design. Male collegiate athletes underwent four experimental sessions; one involving no potentiation warm up, one involving a biomechanically dissimilar CA, and two involving a biomechanically similar CA (one being sagittal plane movements and one being transverse/frontal plane movements) prior to a high velocity movement performance task. The subjects were tested on a rotational medicine ball throw and vertical jump as the HVM following the warmup conditions. Force-velocity measures were taken during the HVM to determine the potentiation effect of the different testing conditions. The results of this study showed a significant increase in multiple force variables (peak force, relative peak force, horizontal peak force, and horizontal rate of force development) of both the back leg and lead leg in a transverse plane HVM following a transverse plane CA, or biomechanically similar action, confirming existing literature. However, no significant increase in transverse HVM was seen following a sagittal CA, i.e. when the HVM was in a biomechanically dissimilar plane of motion. These results suggest that potentiation is dependent on the biomechanical aspects of the CA used.