The current study examined the relationship between witnessing violence and racial discrimination on depressive symptoms and accelerated epigenetic aging in a non-treatment seeking sample of Black Americans. In addition to the direct effects of witnessing violence and experiences of racial discrimination on depressive symptoms and accelerated epigenetic aging, analyses also assessed whether experiences of racial discrimination moderated the impact of witnessing violence on depressive symptoms and accelerated epigenetic aging. Partially in line with our hypotheses, witnessing violence and racial discrimination were found to predict depressive symptoms, but not accelerated epigenetic aging. Although no modifying effect was found for accelerated epigenetic aging, racial discrimination exacerbated the relationship between witnessing violence and depressive symptoms. This study adds to the current body of knowledge by identifying sociocultural factors that contribute to the well-being of Black Americans, and likely play a role in the persistent racial health inequalities experienced by Black Americans.