Hospital-acquired infections is a major public health crisis in the United States. The burden on healthcare workers to eliminate recontamination through repeated cleaning has highlighted the need for continuously active disinfectants against common healthcare pathogens. The study aims to identify proper solutions and methods for surface sampling to detect contamination after disinfecting with Sani-24, a continuous active disinfectant. The sampling solutions and methods tested this study include: Neutralizing Buffer (sodium monopotassium, sodium thiosulfate, aryl sulfonate complex), Eluent (PBS+0.01 % Tween 80), and Cellulose Sponge Stick recovery. When testing MRSA exposure to neutralizing buffer, eluent, and cellulose, no relationship between the bacterial log reduction and time was detected. This showed that the proposed surface sampling methods did not kill the bacteria of interest throughout an extend time, as intended.