Date of Award
Master of Science (MS)
Dr. Hamed Laroui
IBD (inflammatory bowel disease) is a pandemic chronic disease of the gastrointestinal tract inducing Ulcerative colitis (UC) and Crhon disease (CD). The inflammation of colon especially associated with the growing risk of developing colon cancer. To extend the research on IBD, chemical mice models are investigated on easy way to induce colitis in rodent. Dextran sulfate salt (DSS) 7 days, 3% in drinking water induce IBD on mice. One of the problem of DSS is that it requires a purification step of mRNA by LiCl. This step induce this mRNA harvest rate quantitatively and qualitatively.
In this study, we synthesized Pullulan Sulfate and Cyclodextrin Sulfate as alternative colitis inducers in mice. The specificity of sulfated polysaccharides in colon versus small intesinte has been confirmed by common gastrointestinal method (pro inflammatory cytokines causes degradation of the epithelium,and the presence of blood in stool.)
INDEX WORDS: IBD, mice model, DSS, mRNA purification, Pullulan and Cyclodextrin.
Ma, Qiang, "synthesis of sulfated polysaccharides for colitis mice model." Thesis, Georgia State University, 2015.