Title
Each of the Eight Simian Hemorrhagic Fever Virus Minor Structural Proteins is Functionally Important
Document Type
Article
Publication Date
7-2014
Abstract
The simian hemorrhagic fever virus (SHFV) genome differs from those of other members of the family Arterivirus in encoding two adjacent sets of four minor structural protein open reading frames (ORFs). A stable, full-length, infectious SHFV-LVR cDNA clone was constructed. Virus produced from this clone had replication characteristics similar to those of the parental virus. A subgenomic mRNA was identified for the SHFV ORF previously identified as 2b. As an initial means of analyzing the functional relevance of each of the SHFV minor structural proteins, a set of mutant infectious clones was generated, each with the start codon of one minor structural protein ORF mutated. Different phenotypes were observed for each ortholog of the pairs of minor glycoproteins and all of the eight minor structural proteins were required for the production of infectious extracellular virus indicating that the duplicated sets of SHFV minor structural proteins are not functionally redundant.
Recommended Citation
Heather A. Vatter, Han Di, Eric F. Donaldson, Ralph S. Baric, Margo A. Brinton. Corrigendum to “Each of the eight simian hemorrhagic fever virus minor structural proteins is functionally important” [Virology 462–463 (2014) 351–362] Virology, Volumes 464–465, September 2014, Page 461. doi: http://dx.doi.org/10.1016/j.virol.2014.06.001
Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Comments
Originally Published in:
Virology, 462-463 351-62. doi: http://dx.doi.org/10.1016/j.virol.2014.06.001