Date of Award
Fall 1-7-2011
Degree Type
Thesis
Degree Name
Master of Science (MS)
Department
Biology
First Advisor
John E. Houghton
Second Advisor
Julia K. Hilliard
Third Advisor
William Walthall
Abstract
Saccharomyces cerevisiae exhibits an apoptotic response upon exposure to toxic metals such as cadmium (Cd) and copper (Cu). Preliminary findings indicate that this response is dependent –to some extent- on the presence of a fermentable carbon source, glucose. To investigate this dependency we monitored the apoptotic response to both metals in the presence and absence of glucose and have shown that glucose is absolutely necessary in order to induce apoptosis in yeast at least during the exposure to metal. We have also looked at the biochemical changes that are taking place in yeast when treated with Cd using Fourier Transform Infra-Red (FTIR) Spectroscopy. Our results suggest that there are definitive changes in cellular activities that are discernable at 1660-1640cm-1 (amide I), 1540-1510cm-1 (amide II) and 1140-1080cm-1(DNA absorption bands).
DOI
https://doi.org/10.57709/1743218
Recommended Citation
Koduru, Rupa, "Study of Cellular Activities in Response to Metal-Induced Apoptosis in Saccharomyces Cerevisiae using FTIR." Thesis, Georgia State University, 2011.
doi: https://doi.org/10.57709/1743218