Date of Award

Fall 12-7-2011

Degree Type

Thesis

Degree Name

Master of Science (MS)

Department

Biology

First Advisor

Casonya Matese Johnson

Second Advisor

Walter (Bill) William Walthall

Third Advisor

Parjit Kaur

Abstract

Notch signaling is important for development in Caenorhabditis elegans and the REF-1 family pro-teins, a set of the bHLH transcription factors, are the first targets of Notch signaling. Little is known about the molecular mechanisms employed by the REF-1 family to regulate development. In this project, I iden-tified novel targets of three REF-1 family proteins, HLH-25/HLH-28/HLH-29, and determined which target genes are activated and which are repressed by the REF-1 proteins. These targets were identified by gene expression microarray and were functionally categorized by Gene Oncology analysis. A systems biology approach was performed to identify networks associated with those targets. In addition to the mo-lecular genetics studies, I identified and better characterized the range of phenotypes induced by muta-tions in ref-1 family genes.

DOI

https://doi.org/10.57709/2391945

Supplementary Tables S1234.xlsx (83 kB)
Supplementary Tables S1234

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