Title
Cell-Type Specific Transcriptomic Profiling to Dissect Mechanisms of Differential Dendritogenesis
Document Type
Article
Publication Date
10-2014
Abstract
The establishment, maintenance and modulation of cell-type specific neural architectures are critically important to the formation of functional neural networks. At the neuroanatomical level, differential patterns of dendritic arborization directly impact neural function and connectivity, however the molecular mechanisms underlying the specification of distinct dendrite morphologies remain incompletely understood. To address this question, we analyzed global gene expression from purified populations of wild-type class I and class IV Drosophila melanogaster dendritic arborization (da) sensory neurons compared to wild-type whole larval RNA using oligo DNA microarray expression profiling. Herein we present detailed experimental methods and bioinformatic anal- yses to correspond with our data reported in the Gene Expression Omnibus under accession number GSE46154. We further provide R code to facilitate data accession, perform quality controls, and conduct bioinformatic analyses relevant to this dataset. Our cell-type specific gene expression datasets provide a valuable resource for guiding further investigations designed to explore the molecular mechanisms underlying differential patterns of neuronal patterning.
Recommended Citation
Bhattacharya, Surajit, Eswar Prasad R. Iyer, Srividya Chandramouli Iyer, and Daniel N. Cox. “Cell-Type Specific Transcriptomic Profiling to Dissect Mechanisms of Differential Dendritogenesis.” Genomics Data 2 (December 2014): 378–81. doi: http://dx.doi.org/10.1016/j.gdata.2014.10.011.
Creative Commons License
This work is licensed under a Creative Commons Attribution-Noncommercial-No Derivative Works 3.0 License.
Comments
Originally Published in:
Genom Data, 2 378-381. DOI: http://dx.doi.org/10.1016/j.gdata.2014.10.011