Document Type
Article
Publication Date
10-2014
Abstract
Numerous in vivo functional studies have indicated that the dimeric extracellular domain (ECD) of the CaSR plays a crucial role in regulating Ca2+ homeostasis by sensing Ca2+ and L-Phe. However, direct interaction of Ca2+ and Phe with the receptor’s ECD and the resultant impact on its structure and associated conformational changes have been hampered by the large size of the ECD, its high degree of glycosylation, and the lack of biophysical methods to monitor weak interactions in solution. In the present study, we purified the glycosylated extracellular domain of CaSR (ECD) (residues 20~612), containing either complex or high mannose N-glycan structures depending on the host cell line employed for recombinant expression. Both glycosylated forms of the CaSR ECD were purified as dimers and exhibit similar secondary structures with ~50% -helix, ~20% -sheet content and a well buried Trp environment. Using various spectroscopic methods, we have shown that both protein variants bind Ca2+ with a Kd of 3.0~5.0 mM. The local conformational changes of the proteins induced by their interactions with Ca2+ were visualized by NMR with specific 15N Phe-labeled forms of the ECD. Saturation transfer difference (STD) NMR approaches demonstrated for the first time a direct interaction between the CaSR ECD and L-Phe. We further demonstrated that L-Phe increases the binding affinity of the CaSR ECD for Ca2+. Our findings provide new insights into the mechanisms by which Ca2+ and amino acids regulate the CaSR and may pave the way for exploration of the structural properties of CaSR and other members of family C of the GPCR superfamily.
Recommended Citation
Direct Determination of Multiple Ligand Interactions with the Extracellular Domain of the Calcium Sensing Receptor. Chen Zhang, You Zhuo, Heather A. Moniz, Shuo Wang, Kelley W. Moremen, James H. Prestegard, Edward M. Brown and Jenny J. Yang. J. Biol. Chem. published online October 10, 2014. doi: http://dx.doi.org/10.1074/jbc.M114.604652
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Originally Posted in:
J Biol Chem, 289 (48), 33529-42. doi: http://dx.doi.org/10.1074/jbc.M114.604652