Date of Award
Summer 5-20-2011
Degree Type
Thesis
Degree Name
Master of Science (MS)
Department
Chemistry
First Advisor
Dr. Kathryn B. Grant
Second Advisor
Dr. Dabney W. Dixon
Third Advisor
Dr. Donald Hamelberg
Abstract
DNA photosensitizers are compounds that are capable of binding in to DNA strands through groove binding, intercalation, or electrostatic interactions. Excitation of these agents by light generates reactive oxygen species which causes extensive photo-oxidative damage to genomic DNA. Physiological concentrations of NaCl and KCl are ~ 150 mM and 260 mM within the cell nucleus where DNA is contained. Unfortunately, the ability of most photosensitizers to bind to double-helical DNA is reduced and photocleavage yields are diminished as concentrations of salt increase. The aim of this project is to observe the photocleavage of pUC19 plasmid DNA induced by N1,N1-bis(9-anthrymethyl)triethylenetetraamine tetrahydrochloride (AL-VIII 23) 1 or N,N-dimethyl-N’-(9-methylanthracenyl)ethylenediamine (NMEA) 2 in presence of salt. Spectroscopic titrations and DNA melting assays were used to study binding modes and affinities of both dyes to the helix upon the addition of salt.
DOI
https://doi.org/10.57709/2041941
Recommended Citation
Deeyaa, Blessing D., "DNA Photocleavage by 9-Aminomethylanthracene Dyes at pH 7.0: Ionic Strength Effects." Thesis, Georgia State University, 2011.
doi: https://doi.org/10.57709/2041941